Electrospray Ionization Mass Spectrometry
& Studies of Noncovalent Interactions

Overview:

Since its invention in 1984 by J. B. Fenn electrospray ionization mass spectrometry (ESI-MS) has found increasing use for binding constant determination. Today there are numerous examples published, in which this soft ionization method was used successfully for determining the binding constants of noncovalently bound complexes . However, it is still under debate if and for which conditions ESI-MS gives accurate and reliable values for binding constants. We are working on a better understanding of the fundamentals for the detection of noncovalent complexes with ESI-MS. This knowledge is also used to further develop mass spectrometry based methods to approach biochemical problems.

 

Projects:
 
     
 
Determination of binding constants with mass spectrometry
 
Influence of the electrospray process on chemical equilibria
 
Nuclear/Hormone receptors (completed)
     
 

 

Personnel:

Konstantin Barylyuk has a diploma in biochemistry from Moscow State University where he used mass spectrometry to study membrane protein topology. He joined our noncovalent interactions team in January 2009. In his spare time he enjoys hiking and Russian baths.
Rui Wang has started her Ph.D in September 2007. Coming from Tsinghua University she has a strong background in spectroscopy and numerical modeling that she is using to study electrospray ionization processes.
Dr Elisabetta Boeri Erba received her Ph.D. degree from the University of Turin in collaboration with the University of Southern Denmark. During her Ph.D. studies, she developed MS-based methods to investigate protein phosphorylation. As a postdoc in the group of Prof Carol Robinson (Cambridge, UK), she studied non-covalent protein complexes by ESI. In April 2010 Elisabetta joined our group to study non-covalent interactions by ESI- and MALDI-mass spectrometry
Dragana Cubrilovic received her master degree in chemistry from the Goethe University Frankfurt a.M. At the group of Prof. Michael Karas she worked on Ultra-thin layer prepared matrix compounds for the MALDI-MS analysis of high-molecular proteins. Dragana started her PhD in March 2011 to study noncovalent interactions by mass spectrometry.

 

 

 


  
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